Product category
品牌 | 其他品牌 | 貨號 | CPCR-0005/CPCR-0050/CPCR-0500 |
---|---|---|---|
規(guī)格 | 5 mL/50 mL/500 mL | 供貨周期 | 一周 |
主要用途 | 高效純化PCR擴增產(chǎn)物 |
紅榮微再(上海)生物工程技術(shù)有限公司 :1500 1904 520。紅榮微再-客服: 經(jīng)銷商專員
CleanPCR磁珠是歐洲品牌CleanNA的DNA提取純化試劑盒,是基于順磁技術(shù)的高效PCR和Next Generation文庫制備純化系統(tǒng),可高效純化PCR擴增產(chǎn)物。憑借其簡單的三步法,CleanPCR使DNA片段選擇性地結(jié)合到磁珠上,同時去除鹽,引物,引物二聚體和dNTPs; 高度純化的DNA用低鹽洗脫緩沖液或水洗脫,可直接用于下游應(yīng)用。CleanPCR方法可以使用手工操作,也可以適用于自動化工作站的現(xiàn)有方法(例如Beckman,Hamilton,Tecan,Caliper,Perkin Elmer,Agilent和Eppendorf)。
The CleanPCR kit is an efficient PCR and Next Gen library prep clean up system based on paramagnetic beads technology, providing an efficient purification of PCR amplicons. With its simple, three-step protocol, CleanPCR removes salts, primers, primer-dimers, dNTPs, while DNA fragments are selectively bound to the magnetic beads particles; and highly purified DNA is eluted with low salt elution buffer or water and can be used directly for downstream applications. The protocol can be adapted to your current liquid handling workstation (e.g. Beckman, Hamilton, Tecan, Caliper, Perkin Elmer, Agilent and Eppendorf) utilizing your current protocol as well as it can be performed manually.
CleanPCR特征:
•高于100 bp的擴增子具備高回收率
•有效去除dNTPs,引物,引物二聚體和其他雜質(zhì)
•純化后PCR產(chǎn)物具有穩(wěn)定性和高回收率
•不需要離心或過濾
用CleanPCR系統(tǒng)純化的擴增子可立即用于以下應(yīng)用:
•PCR
•突變檢測和基因分型
•測序(Sanger和NGS)
•片段分析
•微陣列
•限制性酶純化
•克隆
CleanPCR訂購信息
品牌 | 貨號 | 產(chǎn)品描述 | 反應(yīng)數(shù)量(96孔)* |
CleanNA | CPCR-0005 | CleanPCR (5 mL) | 277 |
CleanNA | CPCR-0050 | CleanPCR (50 mL) | 2.777 |
CleanNA | CPCR-0500 | CleanPCR (500 mL) | 27.777 |
*基于 20 µl PCR反應(yīng)量
CleanNGS 是CleanPCR的更新版
CleanNGS的新功能和優(yōu)化性能
- 產(chǎn)品更新適配文庫構(gòu)建方法
- *的片段篩選效率
- 更高的磁含量,更快的分離時間
- 生產(chǎn)過程無RNase,可用于處理RNA
CleanNGS magnetic beads訂購信息
品牌 | 貨號 | 產(chǎn)品描述 | 規(guī)格(反應(yīng)次數(shù))* |
CleanNA | CNGS-0005 | CleanNGS(5 mL) | 277 |
CleanNA | CNGS-0050 | CleanNGS(50 mL) | 2,777 |
CleanNA | CNGS-0500 | CleanNGS(500 mL) | 27,777 |
*基于10 µl PCR反應(yīng)量
自備材料
•乙醇 •CleanNA環(huán)形磁鐵板 •用于DNA洗脫的TE或試劑級水