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品牌 | Stemcell | 貨號(hào) | 18085,18085RF |
---|---|---|---|
規(guī)格 | Kit | 供貨周期 | 一個(gè)月 |
主要用途 | 高純度NK細(xì)胞分離 |
英文名稱(chēng):EasySep™ Human Buffy Coat CD56 Positive Selection Kit
中文名稱(chēng):EasySep人白膜層CD56正選試劑盒
品牌:StemCell
貨號(hào):18085,18085RF
用途:高純度NK細(xì)胞分離
EasySep人白膜層CD56正選試劑盒優(yōu)點(diǎn)
• 快速和即用型
• 高達(dá)99%的純度
• 不需要色譜柱
EasySep™Human Buffy Coat CD56正選試劑盒旨在通過(guò)正選從新鮮制備的白膜層中分離CD56 +陽(yáng)性細(xì)胞。四聚體抗體復(fù)合物識(shí)別CD56和葡聚糖包被的磁珠,以此來(lái)識(shí)別目標(biāo)細(xì)胞。混合液還含有針對(duì)人Fc受體的抗體以使非特異性結(jié)合zui小化。使用EasySep™磁體將標(biāo)記的細(xì)胞分離出來(lái),無(wú)需使用色譜柱。目標(biāo)細(xì)胞保留在管中,同時(shí)不需要的細(xì)胞被倒出。 CD56抗原在NK細(xì)胞和NKT細(xì)胞上表達(dá)。
如果希望分離速度更快,可以選擇EasySep™ HLA Chimerism Buffy Coat CD56 Positive Selection Kit (17875)。
EasySep™ Human Buffy Coat CD56 Positive Selection Kit
Immunomagnetic positive selection cell isolation kit
The EasySep™ Human Buffy Coat CD56 Positive Selection Kit is designed to isolate CD56+ cells from freshly prepared buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD56 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD56 antigen is expressed on NK cells and NKT cells.
分離樣本:30mL白膜層
分離細(xì)胞純度:89.7 - 99.8%
細(xì)胞類(lèi)型:NK細(xì)胞
物種:人
樣本來(lái)源:白膜層
選擇方法:正選
應(yīng)用:細(xì)胞分離
型號(hào):EasySep; RoboSep
研究領(lǐng)域:嵌合體; HLA; 免疫學(xué)
組份:正選試劑盒包括四聚體抗體混合物,磁珠和紅細(xì)胞裂解液。
保存溫度:2-8℃
配套設(shè)備:EasySep磁極,貨號(hào) 18000。
參考文獻(xiàn) (1)
Cell 2016 SEP.A Functional Role for Antibodies in Tuberculosis.Lu LL et al.
NK細(xì)胞磁珠分離系列產(chǎn)品(StemCell)
加拿大StemCell公司主營(yíng)細(xì)胞培養(yǎng)基及添加物、細(xì)胞分選產(chǎn)品、原代及培養(yǎng)細(xì)胞、細(xì)胞凍存液、細(xì)胞因子等。
人NK細(xì)胞分離:正選CD56+陽(yáng)性
白膜層來(lái)源人NK細(xì)胞正選磁珠
貨號(hào) | 產(chǎn)品描述 | 規(guī)格 | 型號(hào) | |
18085 | Buffy Coat EasySep CD56 Positive Kit | 1 EA | EASYSEP | |
18085RF | RoboSep Human CD56 Selection Buffy Coat | 1 EA | ROBOSEP-H-POS-W | |
18085HLA | EasySep HLA BC CD56 Positive Slctn Kit | 1 Kit | EASYSEP | |
18085HLARF | RoboSep HLA BC CD56 Pos Slctn Kit | 1 Kit | ROBOSEP-H-POS |
外周血單個(gè)核細(xì)胞來(lái)源人NK細(xì)胞正選磁珠
17855 | EasySep Human CD56 Pos Sel Kit II | 1 Box | EASYSEP |
17855RF | RoboSep Human CD56 Pos Sel Kit II | 1 Kit | ROBOSEP-H-POS |
人NK細(xì)胞分離:負(fù)選
外周血單個(gè)核細(xì)胞來(lái)源人NK細(xì)胞負(fù)選磁珠
17955 | EasySep Human NK Cell Iso Kit | 1 Box | NEG-EASYSEP-H |
17955RF | RoboSep Human NK Cell Iso Kit | 1 Kit | ROBOSEP-H-NEG |
全血來(lái)源人NK細(xì)胞負(fù)選磁珠
19665 | EasySep Direct Hu NK Iso Kit | 1 Box | NEG-EASYSEP-H |
15025 | RosetteSep Human NK Cells, 2mL vial(s) | 1 Box | ROSETTESEP |
15065 | RosetteSep Human NK Cells, 10mL | 1 Box | ROSETTESEP |
小鼠NK細(xì)胞分離:脾臟來(lái)源小鼠NK細(xì)胞正選和負(fù)選
正選
18755 | EasySep Mouse CD49b Pos Sel Kit | 1 Box | EASYSEP-M |
18755RF | RoboSep Mouse CD49b PosSel Flt Kit | 1 Kit | ROBOSEP-M-POS |
負(fù)選
19855 | EasySep Mouse NK Cell Isolation Kit | 1 Box | NEG-EASYSEP-M |
19855RF | RoboSep Mouse NK Cell Isolation Kit | 1 Kit | ROBOSEP-M-NEG |
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