簡要描述：Dynabeads免疫磁珠Human T-Activator CD3/CD28：
Dynabeads™ Human T-Activator CD3/CD28 免疫磁珠通過偶聯CD3/CD28單抗，活化擴增人T細胞。

Dynabeads™ Human T-Activator CD3/CD28 免疫磁珠通過偶聯CD3/CD28單抗，活化擴增人T細胞。

Dynabeads™ Human T-Activator CD3/CD28 免疫磁珠

產品英文名 Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation

產品中文名 Dynabeads™ Human T-Activator CD3/CD28 免疫磁珠

產品介紹  Dynabeads® Human T-Activator CD3/CD28 用于活化和擴增人 T 細胞和 T 細胞克隆，以作研究之用。Dynabeads® CD3/CD28 T Cell Expander 提供了活化和擴增 T 細胞的簡單方法，無需抗原呈遞細胞或抗原。 Dynabeads® Human T-Activator CD3/CD28 是均勻的 4.5 µm 超順磁珠，與抗 CD3 和抗 CD28 單克隆抗體的優化混合物偶聯。 通過使抗 CD3 和抗 CD28 抗體與 Dynabeads® 結合，這種微珠可以提供 T 細胞活化和擴增所需的原始和共刺激信號。

Dynabeads免疫磁珠

歡迎您致電 華雅再生醫學旗艦公司：紅榮微再（上海）生物工程技術有限公司  ：1500 1904 520。紅榮微再-客服:  經銷商專員

起始樣品

Dynabeads免疫磁珠適用來自全血或血沉棕黃層的 MNC/PBMC，或者包含 CD3+ T 細胞、CD4+ 或 CD8+ T 細胞、調節性 T 細胞或 T 細胞克隆的免疫系統T 細胞亞群。

擴增效果

人T細胞擴增：12天擴增3000倍

規格

Cell Type: T Cells (Whole Population)

Clonality: Monoclonal

Final Product: Cells

Product Line: DYNAL®, Dynabeads®

Reactivity: Human

Sample Type (Specific): Cell Cultures, PBMC

Target Species: Human

Shipping Condition: Room Temperature

細胞類型：免疫T細胞

克隆：單克隆

產物：細胞

產品線：DYNAL®, Dynabeads®

反應性：人

樣本類型：細胞培養物，PBMC（外周血單個核細胞）

靶種屬：人

運輸條件：室溫

內容及儲存

Dynabeads® Human T-Activator CD3/CD28 are supplied as a suspension containing 4 × 10⁷ Dynabeads®/ml in phosphate buffered saline (PBS), pH 7.4, with 0.1% human serum albumin (HSA). Store at: 2-8°C.

Dynabeads免疫磁珠是一種懸液，4 × 10⁷ Dynabeads®/ml的磁珠溶于PBS，pH 7.4，另含0.1%人白蛋白。

2-8°C儲存。

Dynabeads免疫磁珠特點

Dynabeads® Human T-Activator CD3/CD28 are for the activation and expansion of human T cells. Advantages of Dynabeads® Human T-Activator CD3/CD28:

• Activation of T cells without the need for feeder cells

T細胞活化無需滋養層細胞

• Activated cells that retain in vivo-like function

活化細胞功能同體內培養

• Maximum reproducibility without contamination by soluble antibodies or mitogens

zui高可重復性，沒有抗體溶解和絲裂原污染。

• From 100- to 1,000-fold T cell expansion

T細胞擴增100-1000倍

特別應用

CAR-T細胞活化擴增：

CAR-T免疫療法

繼諾華和Kite公司兩款CAR-T細胞治療被FDA批準，2017年被媒體評為CAR-T療法的元年。

CAR T細胞免疫療法中，因為純化自體抗原呈遞細胞（APCs）用于T細胞活化需要好幾個額外步驟，增加獲得CAR T細胞產品強度和難度。因此研究者開發了另外一種更標準化、更具效率的T細胞活化方法，比如使用抗CD3/抗CD28單克隆抗體（anti-CD3/anti-CD28 monoclonal antibodies）。

而Dynabeads® Human T-Activator CD3/CD28 這種微珠可以提供 T 細胞活化和擴增所需的原始和共刺激信號，通過使抗 CD3和抗 CD28 抗體與 Dynabeads® 結合，更加速擴增。

推薦PeproTech抗CD3/抗CD28單克隆抗體產品

T細胞活化抗人CD3單克隆抗體（Functional，clone: OKT3）可以與TCR復合物結合，激活T細胞。SAFIRE(Sodium Azide Free Immensely Reduced Endotoxins) 類產品是BioGems品牌下的功能性抗體產品。

歡迎您致電 華雅再生醫學旗艦公司：紅榮微再（上海）生物工程技術有限公司  ：1500 1904 520。紅榮微再-客服:  經銷商專員

About Dynabeads® Human T-Activator CD3/CD28

Dynabeads® Human T-Activator CD3/CD28 offer a simple method for activation and expansion of T cells that does not require feeder cells (antigen-presenting cells) or antigen. The uniform 4.5 µm diameter, inert, superparamagnetic beads are similar in size to antigen-presenting cells, and are covalently coupled to anti-CD3 and anti-CD28 antibodies. These two antibodies provide primary and co-stimulatory signals, optimized for efficient T cell activation and expansion. Expansion of the T cell population can be stimulated using recombinant IL-2, and after activation or expansion, the magnetic beads can be easily removed using a DynaMag™ magnet. For expansion of antigen-specific T cells from T cell clones or T cell lines, we recommend using Dynabeads® Human T-Activator CD3/CD28/CD137.

Starting samples

Start with mononuclear cells (MNCs)/peripheral blood mononuclear cells (PBMCs) from either whole blood or buffy coat, or T cell subsets including CD3+ T cells, CD4+ or CD8+ T cells, or regulatory T cells.

Downstream applications

The activated T cells can be analyzed shortly after activation (for transfection/transduction or to study TCR signaling, proteomics, gene expression, etc.). T cells can be left in culture to differentiate into T helper cell subsets or expansion of polyclonal/antigen-specific T cells.

For Research Use Only. Not for use in diagnostic procedures.

引用及參考文獻

Activated human CD4+ T cells express transporters for both cysteine and cystine.

Levring TB, Hansen AK, Nielsen BL, Kongsbak M, von Essen MR, Woetmann A, Odum N, Bonefeld CM, Geisler C, Sci Rep (2012) 2:266-266

Dasatinib, a small molecule protein tyrosine kinase inhibitor, inhibits T cell activation and proliferation.

Schade AE, Schieven GL, Townsend R, Jankowska AM, Susulic V, Zhang R, Szpurka H, Maciejewski JP, Blood 2007 0(0):-

Loss of Siglec expression on T lymphocytes during human evolution.

Nguyen DH, Hurtado-Ziola N, Gagneux P, Varki A

Proc Natl Acad Sci U S A (2006) 103:7765-7770