簡(jiǎn)要描述：Definitive Endoderm Cells內(nèi)胚層干細(xì)胞是從Cellartis 人iPS細(xì)胞株ChiPSC18經(jīng)體外誘導(dǎo)分化得來。該產(chǎn)品中，大于90%的細(xì)胞顯示表達(dá)SOX17蛋白。可用于進(jìn)一步分化成為肝細(xì)胞或胰臟細(xì)胞。

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Cellartis定型內(nèi)胚層干細(xì)胞

產(chǎn)品英文名稱：Cellartis Definitive Endoderm Cells (from ChiPSC18)

產(chǎn)品中文名稱：Cellartis定型內(nèi)胚層干細(xì)胞

適合用于進(jìn)一步誘導(dǎo)分化成為肝臟細(xì)胞類型或是胰臟細(xì)胞類型。

Cellartis定型內(nèi)胚層干細(xì)胞（DE cell）是從hiPS細(xì)胞株ChiPSC18經(jīng)體外誘導(dǎo)分化得來。該產(chǎn)品中，大于90%的細(xì)胞顯示表達(dá)SOX17蛋白。該產(chǎn)品為低溫冷凍保存的細(xì)胞懸浮形式。本產(chǎn)品適合用于進(jìn)一步誘導(dǎo)分化成為肝臟細(xì)胞類型或是胰臟細(xì)胞類型。特別地，Cellartis的肝臟細(xì)胞誘導(dǎo)分化試劑盒（Cellartis Hepatocyte Differentiation Kit，Cat. No. Y30050）適合用于DE向肝臟細(xì)胞類型的誘導(dǎo)分化工作。

在產(chǎn)生Cellartis定型內(nèi)胚層細(xì)胞（來自ChiPSC18）的分化過程中，通過RT-qPCR測(cè)量的mRNA表達(dá)譜顯示內(nèi)胚層標(biāo)志物SOX17，F(xiàn)OXA2，F(xiàn)ZD5，CXCR4，HHEX和SOX7的預(yù)期時(shí)間表達(dá); 中胚層標(biāo)記Brachyury; 和多能標(biāo)記OCT4。

Cellartis Definitive Endoderm Cells (from ChiPSC18) are human definitive endoderm (DE) cells derived from the human induced pluripotent stem (hiPS) cell line ChiPSC18. The hiPS cells have been differentiated into definitive endoderm cells in vitro to generate a population of >90% SOX17-protein-expressing cells; they are then dissociated into a cell suspension and cryopreserved. These DE cells are suitable for further differentiation into hepatocytes or pancreatic endoderm cells. To differentiate the DE cells into hepatocytes, use the Cellartis Hepatocyte Differentiation Kit (Cat. No. Y30050).

During the differentiation process that creates Cellartis Definitive Endoderm Cells (from ChiPSC18), the mRNA expression profile—as measured by RT-qPCR—shows the expected temporal expression of endodermal markers SOX17, FOXA2, FZD5, CXCR4, HHEX, and SOX7; mesoendodermal marker Brachyury; and pluripotency marker OCT4. See the Images & Data tab, below.

產(chǎn)品特點(diǎn):

具有> 90％SOX17陽(yáng)性細(xì)胞的定形內(nèi)胚層細(xì)胞

可用于下一步分化內(nèi)胚層起源的下級(jí)分化細(xì)胞，如肝細(xì)胞或胰腺內(nèi)胚層

Definitive endoderm cells with >90% SOX17-positive cells

Can be used for generating further-specified cells of endodermal origin, such as hepatocytes or pancreatic endoderm

組份：

Cellartis Definitive Endoderm Cells (from ChiPSC18) (Cat. No. Y10040)

保存：Cellartis Definitive Endoderm Cells (from ChiPSC18) should be stored at ≤ –150°C. Under

recommended storage conditions, the cells can be stored for up to one year from the date of receipt.

Culture thawed Cellartis Definitive Endoderm Cells (from ChiPSC18) at 37°C ± 1°C, 5% CO2 and>90% humidity.

產(chǎn)品性能：

Immunocytochemistry of Cellartis Definitive Endoderm Cells (from ChiPSC18). Cells were thawed, cultured for two days, fixed, and stained for the pluripotency marker OCT4, the definitive endoderm marker SOX17, and the nuclear marker DAPI. Results show that more than 90% of the cell population are definitive endoderm cells and have lost pluripotency.

相關(guān)產(chǎn)品：

Cellartis DEF-CS™ 500 COAT-1 (Takara Bio, Cat. No. Y30012)

iMatrix-511 (Takara Bio, Cat. No. T303)

iMatrix-511 (Takara Bio, Cat. No. T304)

Cellartis Hepatocyte Differentiation Kit (Takara Bio, Cat. No. Y30050)

肝細(xì)胞培養(yǎng)完整解決方案

人iPS誘導(dǎo)分化成肝細(xì)胞全過程的完整試劑盒：Y30055（新產(chǎn)品）

人iPS來源肝細(xì)胞株及*培養(yǎng)基Y10133/Y10134/Y10135（新產(chǎn)品）

人原代肝臟細(xì)胞培養(yǎng)基 Y20020（新產(chǎn)品）

http://www.chem17.com/st321032/article_1496885.html

華雅再生醫(yī)學(xué)旗艦公司：紅榮微再（上海）生物工程技術(shù)有限公司主營(yíng)細(xì)胞治療和精準(zhǔn)醫(yī)療等產(chǎn)品，2018年與TAKARA繼續(xù)合作，授權(quán)代理（上海）其旗下Cellartis干細(xì)胞研究制品和Rubicon單細(xì)胞文庫(kù)構(gòu)建試劑。Cellartis公司位于瑞典，擁有iPS細(xì)胞等干細(xì)胞分化為肝細(xì)胞及臟器細(xì)胞的分化誘導(dǎo)技術(shù)和ES細(xì)胞，ips細(xì)胞，分化細(xì)胞等干細(xì)胞相關(guān)產(chǎn)品。

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