簡要描述：BioWhittaker® X-VIVO™ 04-743Q X-VIVO™ 10 不含酚紅化學成分確定的無血清造血干細胞培養基

產品英文名稱：X-VIVO™ 10 Chemically Defined, Serum-free Hematopoietic Cell Medium
產品中文名稱：X-VIVO™ 10 化學成分確定的無血清造血干細胞培養基

X-VIVO™ Chemically Defined, Serum-free Hematopoietic Cell Medium 研究背景
 Lonza’s R&D efforts and collaboration with many clinical trials have provided Lonza with the tools and expertise to support the developments in adoptive immunotherapy, cancer therapy, genetic therapy and other cellular therapies. Lonza’s philosophy for the development of serum-free formulations for use in cellular therapy is to provide a nutritionally complete and balanced environment for the cells. We have not included any exogenous growth factors, artificial stimulators of cellular proliferation or undefined supplements.
The products are devoid of any protein-kinase C stimulators and are suitable for the investigation of second messenger systems in the activation of human and murine lymphocytes. The formulations are complete and contain pharmaceutical grade human albumin, recombinant human insulin and pasteurized human transferrin. All X-VIVO™ Medium products are manufactured under current GMPs and are listed with the FDA in a product Master File. Permission to cross-reference the Master File may be obtained by contacting the Product Manager.

X-VIVO™ 10 Chemically Defined, Serum-free Hematopoietic Cell Medium 簡介
The X-VIVO™ 10 Medium formulation is designed to support the generation of Lymphokine Activated Killer (LAK) cells in a serum-free environment. The original protocols involved the incubation of patient or normal donor Peripheral Blood Lymphocytes (PBL) at 1.0-3.0 x 106 cells/ml for a period of 3 days in the presence of 1,000 Cetus units of rIL-2/ml. Optimal LAK cell generation is achieved when peripheral blood lymphocytes are incubated for three to ten days at a density of 1.0-6.0 x106 cells/ml in the presence of 100 - 1000 Cetus units of rIL-2. X-VIVO™ 10 Medium is available as a 1X liquid in several convenient configurations.

X-VIVO™ 10 化學成分確定的無血清造血細胞培養基特點
» 不含血清
» 化學成份明確
» 成份包含L-谷氨酰胺，不包含慶大霉素和酚紅
» zui適于無血清環境中腫瘤浸潤淋巴細胞(TIL)增殖

X-VIVO™ 10 Chemically Defined, Serum-free Hematopoietic Cell Medium 使用注意事項
1.The X-VIVO™ 10 formulation was designed to support the generation of Lymphokine Activated Killer (LAK) cells in a serum-free environment.
 2.Please see Supplemental Information for additional hematopoietic cell applications.
 3. X-VIVO™ 10, catalog number 04-743Q contains L-glutamine but does not contain gentamicin or phenol red.

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